Protein–protein interaction
- Cdc24 interacts with septins to create a positive feedback loop during bud site assembly in yeast
Summary: The Cdc42 guanine-nucleotide-exchange factor Cdc24 interacts with septins during bud site assembly. This interaction assists in recruiting septins and stabilizing Cdc24 at the incipient bud site.
- Two conserved glycine residues in mammalian and Dictyostelium Rictor are required for mTORC2 activity and integrity
Summary: Two glycine residues are essential for the mTORC2 activity and integrity in organisms that are distantly related, suggesting they have a conserved role in mTORC2 assembly and functioning.
- High-content tripartite split-GFP cell-based assays to screen for modulators of small GTPase activation
Summary: The development of a fluorescent reporter of GTPase activation based on tripartite split-GFP that enables the evaluation of GEF activity and the effect of modulators of GTPase activation in a high-content analysis.
- Derlin-1 promotes ubiquitylation and degradation of the epithelial Na+ channel, ENaC
Summary: Derlin-1 binds to ENaC and promotes its ubiquitylation and degradation via ER-related degradation. The E3 ligase HUWE1 is required and adds K11-linked ubiquitin chains to ENaC prompting its degradation.
- Calsequestrin interacts directly with the cardiac ryanodine receptor luminal domain
Summary: Direct physical and functional calsequestrin 2 association with the ryanodine receptor luminal domain is the minimal requirement to form the sarcoplasmic reticulum Ca2+ sensor, which governs cardiac Ca2+ homeostasis and reliable heartbeat.
- FoxO1 inhibits transcription and membrane trafficking of epithelial Na+ channel
Summary: FoxO1 is a key negative regulatory factor in the insulin-dependent control of ENaC expression, and FoxO1 phosphorylation promotes ENaC membrane trafficking by binding to 14-3-3ε in epithelium.
- Architectures of multisubunit complexes revealed by a visible immunoprecipitation assay using fluorescent fusion proteins
Highlighted Article: The architectures of the BBSome and exocyst are revealed by a visible immunoprecipitation assay, which can detect interactions between fluorescent fusion proteins without performing western blotting.
- GABAB receptor promotes its own surface expression by recruiting a Rap1-dependent signaling cascade
Summary: GABAB receptor activation induces Rap1 activation to promote its own surface expression by stimulating receptor recycling, thereby providing a potential explanation for the long-lasting action of GABA.