Saccharomyces cerevisiae
- Proteomic analysis reveals the direct recruitment of intrinsically disordered regions to stress granules in S. cerevisiae
Summary: Mass spectrometry analysis reveals a large cohort of novel yeast stress granule proteins with intrinsic disordered regions that are sufficient for recruitment to the membraneless stress granule compartment.
- Spatiotemporal dissection of the trans-Golgi network in budding yeast
Highlighted Article: The TGN displays two sub-stages of maturation: ‘early TGN’, when retrograde traffic is received, and ‘late TGN’, when transport carriers are produced. At the late TGN, various coat/adaptor proteins exhibit distinct assembly dynamics.
- Yeast ceramide synthases, Lag1 and Lac1, have distinct substrate specificity
Highlighted Article: Distinct substrate specificities of Lag1 and Lac1, the two yeast ceramide synthases, are revealed, shedding light on their physiological roles.
- Iron affects Ire1 clustering propensity and the amplitude of endoplasmic reticulum stress signaling
Highlighted Article: To respond to folding stress in the ER, cells activate the conserved sensor Ire1. We show that iron is required for optimal Ire1 activation and suggest this is because iron is required for ergosterol biosynthesis.
- Characterization of proteome dynamics during growth in oleate reveals a new peroxisome-targeting receptor
Highlighted Article: A high-content screen uncovered many changes in protein localization in yeast grown in oleate and highlighted a new condition-specific peroxisomal protein, Pex9, which targets a subset of proteins to peroxisomes.
- Cdc48 and Ubx1 participate in a pathway associated with the inner nuclear membrane that governs Asi1 degradation
Summary: The inner nuclear membrane (INM) E3 ubiquitin ligase component Asi1 is ubiquitylated, removed from the membrane and targeted to nuclear proteasomes through a new INM-associated degradation pathway.
- Clathrin-mediated endocytosis in budding yeast at a glance
Summary: A review of the current knowledge of the timeline of clathrin-mediated endocytosis in the budding yeast S. cerevisiae, including discussion of recent works that focus on how this process is regulated.
- Proteasome storage granules are transiently associated with the insoluble protein deposit in Saccharomyces cerevisiae
Summary: Carbon source depletion in Saccharomyces cerevisiae leads to the triage of proteasome storage granules (PSGs) and dysfunctional proteasomes on the IPOD, to ensure that only structurally ‘correct’ proteasomes are deployed.