Zebrafish
- Cell Tracking Profiler – a user-driven analysis framework for evaluating 4D live-cell imaging data
Summary: Analysis of cell shape and movement from 3D time-lapses is currently very challenging. Here, we develop Cell Tracking Profiler and use it to examine RhoA and myosin II control of muscle stem cell behaviour during zebrafish regeneration.
- The cationic amino acid exporter Slc7a7 is induced and vital in zebrafish tissue macrophages with sustained efferocytic activity
Highlighted Article: The cationic amino acid exporter Slc7a7 is rapidly induced in zebrafish tissue macrophages upon efferocytosis, allowing them to cope with the catabolism of numerous dead cells without compromising their own viability.
- Mitofusin 2 regulates neutrophil adhesive migration and the actin cytoskeleton
Summary: The ER–mitochondria tether maintained by MFN2 fine-tunes Rac activation to regulate neutrophil adhesive migration and adhesion to endothelial cells under shear stress.
- TPC2-mediated Ca2+ signaling is required for axon extension in caudal primary motor neurons in zebrafish embryos
Summary: Characterization of two-pore channel type 2 (TPC2)-mediated Ca2+ release and ADP-ribosyl cyclase 1-like (ARC1-like) activity during axon extension of caudal primary motor neurons (CaPs) in zebrafish embryos.
- Epb41l5 interacts with Iqcb1 and regulates ciliary function in zebrafish embryos
Summary: EPB41L5 has a previously uncharacterized role in controlling ciliary function through IQCB1 and CEP290. This EPB41L5 function appears to be independent of its function in epithelial morphogenesis.
- Neutrophil phagocyte oxidase activity controls invasive fungal growth and inflammation in zebrafish
Summary: Live imaging reveals neutrophil NADPH oxidase activity limits fungal growth and excessive inflammation during Aspergillus nidulans infection in larval zebrafish.
- Heat resilience in embryonic zebrafish revealed using an in vivo stress granule reporter
Summary: Establishment of a new transgenic zebrafish line with knock-in GFP-G3BP1 to visualize stress granule dynamics in live animals in real time.
- Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis
Summary: A translucent zebrafish model allows a window on the pathological angiogenic and fibrotic response to suture implantation and how this can be modulated by genetically or pharmacologically dampening inflammation.
- Nup358 regulates microridge length by controlling SUMOylation-dependent activity of aPKC in zebrafish epidermis
Summary: Microridges on the apical surface of zebrafish peridermal cells are regulated by the conserved polarity kinase aPKC. We show that Nup358-mediated SUMO-modification of aPKC is crucial for this function.