Zebrafish
- Neutrophil phagocyte oxidase activity controls invasive fungal growth and inflammation in zebrafish
Summary: Live imaging reveals neutrophil NADPH oxidase activity limits fungal growth and excessive inflammation during Aspergillus nidulans infection in larval zebrafish.
- Heat resilience in embryonic zebrafish revealed using an in vivo stress granule reporter
Summary: Establishment of a new transgenic zebrafish line with knock-in GFP-G3BP1 to visualize stress granule dynamics in live animals in real time.
- Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis
Summary: A translucent zebrafish model allows a window on the pathological angiogenic and fibrotic response to suture implantation and how this can be modulated by genetically or pharmacologically dampening inflammation.
- Nup358 regulates microridge length by controlling SUMOylation-dependent activity of aPKC in zebrafish epidermis
Summary: Microridges on the apical surface of zebrafish peridermal cells are regulated by the conserved polarity kinase aPKC. We show that Nup358-mediated SUMO-modification of aPKC is crucial for this function.
- Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression
Summary: Knockout of giantin in a genome-engineered cell line and zebrafish models reveals the capacity of the Golgi to control its own biochemistry through changes in gene expression.
- Live imaging reveals distinct modes of neutrophil and macrophage migration within interstitial tissues
Highlighted Article: Neutrophils and macrophages display distinct modes of interstitial migration in vivo. While neutrophils are classic amoeboid cells, macrophages have a distinct morphology and use a more mesenchymal mode of migration in zebrafish larvae.
- A GEF activity-independent function for nuclear Net1 in Nodal signal transduction and mesendoderm formation
Highlighted Article: Net1 associates with Smad2 in the nucleus in a GEF-independent manner and facilitates Smad2 transcriptional activity to guide mesendoderm development in zebrafish.
- Trim33 is essential for macrophage and neutrophil mobilization to developmental or inflammatory cues
Highlighted Article: Trim33, a transcriptional regulator known to modulate TGF-β signalling and hematopoiesis, is essential for the mobilization of macrophages and neutrophils towards developmental or inflammatory cues.
- A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time
Summary: We describe a new bi-directional NF-κB reporter that is significantly more sensitive than other available reporters and permits the detection of the endogenous NF-κB activation at high spatiotemporal resolution in vitro and in vivo.
- A toolbox to study epidermal cell types in zebrafish
Summary: Here we introduce tools to easily track, ablate or monitor subsets of cells in the developing zebrafish epidermis, providing an excellent in vivo model system to study a living epithelium.